https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19753 Sat 24 Mar 2018 07:54:28 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5245 Sat 24 Mar 2018 07:44:19 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:24606 Pecten fumatus was used to test the hypothesis that protracted spawning is supported by both capital and income strategies, depending on the state of energy reserves and food availability at the time of oocyte maturation. The study was carried out in Great Bay, Tasmania, Australia (147.335W, 43.220S) in 2010/2011. The use of glycogen, protein and lipid in the muscle, gonad, and digestive gland was examined, along with the role of atretic eggs as an alternative energy source for oogenesis and maturation. The reproductive stage of an individual was determined using only the ovaries. P. fumatus uses a capital breeding strategy early in the reproductive cycle during winter and spring (August–October) with muscle glycogen and protein and digestive gland lipid providing energy for oogenesis. Given there was no evidence of energy stores being used later in the reproductive cycle in late spring and summer (November–March), when less food was available for direct fuelling of reproduction, it appears that metabolites produced from oocyte lysis may have fuelled oogenesis. Recycling of energy from oocyte resorption must be considered as part of the strategy of energy use to fuel reproduction in marine invertebrates.]]> Sat 24 Mar 2018 07:11:55 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46262 Symbiodinium tridacnidorum and Durusdinium trenchii) using gas chromatography–mass spectrometry (GC–MS) and comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry (GC x GC–TOFMS). Seven chemical classes were detected by both instruments, the most common being aromatic hydrocarbons. However, GC x GC resolved seven times more BVOCs than GC–MS (290 vs. 40), with a higher proportion of compounds tentatively identified (173 vs. 14). Notably, nine chemical classes were exclusively identified by GC x GC, including alkane, alkene, aldehyde, ester, and nitrile BVOCs—each potentially fulfilling undescribed functions in marine organisms. The microalgal species investigated shared a large proportion of BVOCs, and this result was consistent across instruments (97 and 98% shared compounds via GC x GC and GC–MS, respectively), suggesting consistent retrieval of general patterns between instruments. This method comparison is the first of its kind in marine systems and confirms the greater analytical power of GC x GC, required to help resolve complex BVOC emissions and the identification of their roles in marine systems.]]> Mon 14 Nov 2022 15:58:32 AEDT ]]>